Effect of platelet factor 4 on the adhesive property of leukemic CD34+ KG1a cell.
- Author:
Jing ZHANG
1
;
Yue-xia MA
;
Zhong-chao HAN
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD34; metabolism; Cell Adhesion; drug effects; Cell Adhesion Molecules; metabolism; Cell Line, Tumor; Humans; Leukemia, Myeloid, Acute; immunology; pathology; Platelet Factor 4; pharmacology; Umbilical Veins; cytology
- From: Acta Academiae Medicinae Sinicae 2002;24(2):160-164
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of PF4 on the adherence of leukemic CD34+ KG1a cell to human umbilical vein endothelial cell line ECV-304 cell and on the expression of adhesive molecules.
METHODSAdhesion assay and adhesion blocking assay were respectively applied to measure the effect of PF4 and/or adhesion molecule monoclonal antibodies on the adhesion property of KG1a. The expressions of adhesion molecules were determined by RT-PCR and FACS analysis.
RESULTSThe adhesion of KG1a cells to ECV-304 was significantly enhanced in the presence of PF4. Such enhancement was also observed when KG1a or ECV-304 cells were separately treated with PF4 before interaction. The adhesion capacity of KG1a cells was reduced when cells were co-incubated with the blocking monoclonal antibodies (MoAbs) against CD49d, CD106, CD54, respectively. In contrast, MoAbs against CD62L, CD62P and CD62E had no such effect. During a period of 3 hours when KG1a or ECV-304 cells were respectively incubated with PF4, the mRNA expressions of CD49 d, CD54 were up-regulated. Furthermore, when KG1a or ECV-304 cells were incubated with PF4 for 2 hours, respectively, the percentages of CD49d+ KG1a cells and CD54+ ECV-304 were increased significantly.
CONCLUSIONPF4 can enhance KG1a cell adhesive capacity by increasing the expressions of adhesion molecules.