Mutations in the D-loop region of mitochondrial DNA in ovarian tumors.
- Author:
Hong-hui SHI
1
;
Liu VINCENT
;
Ngan HEXTAN
;
Xiu-yu YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cystadenocarcinoma, Serous; genetics; DNA, Mitochondrial; genetics; Female; Humans; Ovarian Neoplasms; genetics; Point Mutation; Polymorphism, Genetic
- From: Acta Academiae Medicinae Sinicae 2002;24(2):170-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate mutations in the D-loop region of mitochondrial DNA in ovarian tumors.
METHODSThe D-loop region of 25 epithelial ovarian tumors together with the adjacent normal tissues were amplified by PCR and sequenced.
RESULTSAmong the 25 ovarian tumors, 26 mutations were identified with the mutation rate of 32%. 19 mutations were detected in two cases of borderline carcinoma which was a special type of epithelial ovarian carcinoma. There were 6 microsatellite instabilities among the mutations and 11 new polymorphisms which were not reported previously in the GenBank.
CONCLUSIONSThe D-loop region of mitochondrial DNA is a highly polymorphoric and mutable region and the mutation rate is relatively high in patients with ovarian tumors.
