The relationship between intracellular chloride concentration and ischemia reperfusion-induced arrhythmias in myocardial cells.
- Author:
Zhong-fang LAI
1
Author Information
1. Department of Pharmacology, School of Medicine, Kumamoto University, Kumamoto 860-0811, Japan. lai-zf@gpo.kumamoto-u.ac.jp
- Publication Type:Journal Article
- MeSH:
Action Potentials;
Animals;
Arrhythmias, Cardiac;
etiology;
metabolism;
Chlorides;
metabolism;
Guinea Pigs;
Hydrogen-Ion Concentration;
Microelectrodes;
Myocardial Reperfusion Injury;
complications;
metabolism;
Myocytes, Cardiac;
metabolism;
Papillary Muscles;
cytology
- From:
Acta Academiae Medicinae Sinicae
2002;24(2):190-196
- CountryChina
- Language:Chinese
-
Abstract:
We investigated the effects of simulated ischemia on intracellular Cl- concentration ([Cl-]i) in guinea pig ventricular myocardial cells and possible role of the [Cl-]i on the ischemia/reperfusion-induced arrhythmias in perfused rat hearts. Our results provided direct evidence that the [Cl-]i in ventricular muscle was increased under ischemic conditions, which suggested that activation of the Cl-(-)HCO3- exchanger by ischemia would partially contribute to the elevation of [Cl-]i. Application of stilbene derivatives or lowering Cl- concentration in perfusion solution delayed the onset of ischemia-induced deterioration in action potentials, pHi, [Cl-]i, and suppressed the incidence of ischemia/reperfusion-induced arrhythmias. The conclusion was made to emphasize the important role of intracellular Cl- homeostasis in cardiac physiology and pathogenesis of myocardial ischemia/reperfusion injury.
