Clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue lymphoma.
- Author:
Wei JIANG
1
;
Gan-di LI
;
Lei LI
;
Yuan TANG
;
Yan-mei HE
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; metabolism; Adult; Aged; B-Cell CLL-Lymphoma 10 Protein; Base Sequence; Cell Nucleus; metabolism; Cell Transformation, Neoplastic; Cloning, Molecular; Cytoplasm; metabolism; Female; Gene Rearrangement, B-Lymphocyte, Heavy Chain; genetics; Humans; Lymphoma, B-Cell, Marginal Zone; genetics; metabolism; pathology; Male; Middle Aged; Oncogene Proteins, Fusion; metabolism; Polymerase Chain Reaction
- From: Chinese Journal of Pathology 2007;36(11):736-741
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue (MALT) lymphoma.
METHODSSix cases of MALT lymphoma with high-grade transformation were studied. Immunohistochemical study was carried out by EliVision using bcl-10 antibody. Reverse transcription-polymerase chain reaction was used to detect the presence of API2-MALT1 fusion gene transcripts. The target cells were selected by laser microdissection and studied by polymerase chain reaction and sequence analysis for rearrangement of immunoglobulin heavy chain gene.
RESULTSIn the 6 cases of MALT lymphoma with high-grade transformation, nuclear and cytoplasmic expression of bcl-10 was demonstrated in 1 case, while API2-MALT1 fusion gene was present in 2 cases. Identical fragments of rearranged immunoglobulin heavy chain gene were detected in both transformed and non-transformed components in each of the 6 cases, except for the differences at 2 nucleotide positions in N or D regions in 2 cases.
CONCLUSIONThe tumor cells from both transformed and non-transformed components in MALT lymphoma derive from the same clone.
