Expression profiles of lipid metabolism-related genes in liver of apoE(-/-)/LDLR(-/-) mice.
- Author:
Hui-qin DU
1
;
Miao YIN
;
Hong-yan YE
;
Yun-ju SHANG
;
Xue-dong DAI
;
Wen JING
;
Liang ZHANG
;
Ning XIAO
;
Ji-feng LI
;
Jie PAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta; pathology; Apolipoprotein A-V; Apolipoprotein B-100; biosynthesis; genetics; Apolipoproteins; biosynthesis; genetics; Apolipoproteins A; biosynthesis; genetics; Apolipoproteins E; deficiency; Atherosclerosis; etiology; metabolism; pathology; CD36 Antigens; biosynthesis; genetics; Gene Expression; Lipid Metabolism; Liver; metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; RNA, Messenger; metabolism; Receptors, LDL; deficiency
- From: Chinese Journal of Pathology 2007;36(11):751-755
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between the expression characteristics of lipid metabolism-related genes in the liver and early atherosclerotic lesions in apolipoprotein E and low density lipoprotein receptor gene double knockout (apoE(-/-)/LDLR(-/-)) mice.
METHODSRT-PCR was used to detect the differential expression of lipid metabolism-related genes in the liver of apoE(-/-)/LDLR(-/-) and wild type (WT) mice. Serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) level as well as aortic morphology were also analyzed.
RESULTSAmong the 11 lipid metabolism-related genes, apolipoprotein B100 (apoB100) mRNA levels were significantly higher in apoE(-/-)/LDLR(-/-)mice compared with WT mice. At 14 days, 1, 2 and 3 months of age, the level of mRNA expression were 1.55, 1.47, 1.50 and 2.42 folds of those of the age matched WT mice respectively. The fatty acid transporter (FAT/CD36) mRNA expression levels were higher in 14-day and 3-month old mice at 1.30 and 1.35 folds of those of the age matched WT mice, respectively. Apolipoprotein A IV (apoA IV) and Apolipoprotein AV (apoAV) mRNA levels were significantly down-regulated (0.89 fold decrease in 14-day, and 0.90 folds decrease in 3-month, respectively). The mRNA expression levels of apolipoprotein AI (apo AI), apolipoprotein F (apo F), peroxidase proliferator-activated receptor alpha (PPAR-alpha), liver X receptor alpha (LXRalpha), angiopoietin-like protein 3 (ANGPTL3), acyl-coenzymeA oxidase 1 (ACOX1) and carnitine palmitoyl transferase 1 (CPT1) had no significant changes. Serum TC, TG and LDL-C were higher than those of age matched WT mice at 7, 2 and 30 folds, respectively. Furthermore, apoE(-/-)/LDLR(-/-) mice demonstrated typical early atherosclerotic lesions at sinus and root regions of aorta in an age dependent manner.
CONCLUSIONAlterations of the expression of lipid metabolism-related genes in liver play important roles in the development of AS in the apoE(-/-)/LDLR(-/-) mice at early ages.
