Induced differentiation of rat hepatic oval cells in-vitro by combined hepatocyte growth factor and epidermal growth factor treatment.
- Author:
Jun LIU
1
;
Ling XUE
;
Meng ZHANG
;
Li-hong CHE
;
Hui-xi WU
;
Rui-de HU
Author Information
- Publication Type:Journal Article
- MeSH: Albumins; metabolism; Animals; Cell Differentiation; drug effects; Epidermal Growth Factor; pharmacology; Glutathione Transferase; biosynthesis; genetics; Hepatocyte Growth Factor; pharmacology; Hepatocytes; cytology; metabolism; ultrastructure; Immunohistochemistry; Keratin-18; metabolism; Protein Array Analysis; Pyruvate Kinase; biosynthesis; genetics; RNA, Messenger; metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Pathology 2007;36(11):756-759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo characterize the biologic featrues of hepatic oval cells and their protein expression profiles during induced differentiation in vitro.
METHODSRat hepatic oval cells were treated with epidermal growth factor (EGF) and hepatocyte growth factor (HGF) in vitro, followed by morphological and molecular marker assessment by electromicroscopy, immunocytochemistry, RT-PCR and protein expression chip technology.
RESULTSTen weeks after induction, the levels of GST-P mRNA and M2-PK mRNA were significantly reduced, whereas those of ALB and CK18 were elevated. Significant variations of expression was seen in 8 protein species during the course of the induced differentiation.
CONCLUSIONCombined EGF and HGF treatment in vitro induces cell differentiation of hepatic oval cells, a process in which 8 protein species may play some regulatory roles.
