Increased expression of extracellular matrix metalloproteinase inducer through interaction between fibroblasts and colonic cancer cells.
- Author:
You-wei ZHANG
1
;
Hong DENG
;
Ping-sheng CHEN
;
Lu-lu CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Basigin; biosynthesis; genetics; Cell Line, Tumor; Cells, Cultured; Coculture Techniques; Colonic Neoplasms; metabolism; pathology; Fibroblasts; cytology; metabolism; Humans; Lung; cytology; RNA, Messenger; metabolism
- From: Chinese Journal of Pathology 2007;36(11):764-767
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of extracellular matrix metalloproteinase inducer gene (EMMPRIN) regulated by the interactions between fibroblasts and colon cancer cells, and to study its role in the invasion and metastasis of colon cancer.
METHODSColon cancer cells (SW480) were co-cultured with fibroblasts (HELF) in RPMI 1640 media for 0, 12, 24 and 48 hours. The expression of EMMPRIN in SW480 cells and HELF cells was documented by RT-PCR and immunocytochemistry.
RESULTSThe mRNA and protein expressions of EMMPRIN in SW480 cells were remarkably increased by the co-culturing with HELF cells. Although without the endogenous expression, HELF cells began to express EMMPRIN in a time-dependent manner after being co-cultured with SW480 cells.
CONCLUSIONSIntercellular interactions between colon cancer cells and fibroblasts not only up-regulate the EMMPRIN expression in SW480 cells, but also induce its expression in HELF cells. Such interactions may play a crucial role in the invasion and metastasis of colon cancer.
