Identification of metastasis-associated proteins of ovarian cancer by proteomics.
- Author:
Le ZHOU
1
;
Xiao-Feng YANG
;
Yan WANG
;
Ying-Tao ZHANG
;
Yi-Ping GENG
;
Lü-Sheng SI
;
Yi-Li WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Electrophoresis, Gel, Two-Dimensional; Female; Gene Expression Regulation, Neoplastic; Humans; Neoplasm Metastasis; Ovarian Neoplasms; metabolism; pathology; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Chinese Journal of Pathology 2007;36(12):814-818
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities.
METHODSThe ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach.
RESULTSThirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system.
CONCLUSIONThe data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.
