Research on distribution and expression of NMDA receptors and parvalbumin-positive neurons in intractable epilepsy-related focal cortical dysplasia.
- Author:
Lei LIU
1
;
Yue-Shan PIAO
;
Wei WANG
;
Li CHEN
;
Li-Feng WEI
;
Hong YANG
;
De-Hong LU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Biomarkers, Tumor; metabolism; Cerebral Cortex; metabolism; pathology; Child; Child, Preschool; Epilepsies, Partial; pathology; Female; Gene Expression Regulation; Humans; Infant; Interneurons; metabolism; Male; Malformations of Cortical Development; pathology; Neurons; metabolism; Parvalbumins; analysis; isolation & purification; Receptors, N-Methyl-D-Aspartate; genetics; metabolism
- From: Chinese Journal of Pathology 2008;37(1):16-20
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the alteration of subunits composition in NMDA receptor and the alterations of the expression and distribution of NMDA receptors and parvalbumin (PV)-positive neurons in focal cortical dysplasia (FCD) cortices.
METHODSTwenty cases of FCD samples (including all four subtypes of FCD) obtained during epilepsy surgery and 4 controls were analysed by immunohistochemical staining for NR1, NR2A/B and PV.
RESULTSIncreased expression of NR1 was detected in the giant neurons and dysmorphic neurons in FCD; while pronounced expression of NR2A/B was detected in immature neurons, giant neurons and dysmorphic neurons of FCD, especially in somata and processes of the immature neurons. Compared with the controls, FCD cortices showed prominent scattered arrangement of PV positive neurons and fibers, dramatically decreased number of PV positive interneurons and PV background staining, especially in foci of FCD II subtype.
CONCLUSIONThere are increased expressions of NR1 and NR2A/B subunits in FCD abnormal neurons, as well as scattered and reduced expressions of PV positive neurons and fibers in FCD cortices.
