Expression of Twist in papillary thyroid carcinomas and its roles in differential diagnosis.
- Author:
Chun-Nian HE
1
;
Lili HE
;
Jin Q CHENG
;
Shi-Chao CHEN
;
Huan-Fen ZHAO
;
Jin-Ping ZHAI
;
Jing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma, Follicular; metabolism; Adenocarcinoma, Papillary; pathology; Adenoma; diagnosis; metabolism; Biomarkers, Tumor; immunology; Carcinoma, Papillary; diagnosis; metabolism; pathology; Carcinoma, Papillary, Follicular; metabolism; Diagnosis, Differential; Galectin 3; genetics; metabolism; Immunohistochemistry; Keratin-19; genetics; Keratins; genetics; metabolism; Nuclear Proteins; genetics; metabolism; Thyroid Neoplasms; diagnosis; metabolism; pathology; Thyroid Nodule; pathology; Twist-Related Protein 1; genetics; metabolism
- From: Chinese Journal of Pathology 2008;37(1):35-39
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study Twist expression in thyroid papillary carcinoma (PTC) by immunohistochemistry and to assess its usefulness as marker in the differential diagnosis of PTC, follicular adenomas (FA) and benign papillary lesions (BPL).
METHODSFifty cases of PTC, 48 cases of FA and 47 cases of BPL were evaluated using manual tissue chip and SP immunohistochemical stain to detect the expression of Twist and HBME-1, and comparing the staining to that of cytokeratin 19 (CK19).
RESULTSIn PTC, positive rates of Twist, HBME-1 and CK19 were 100% (48/48), 94.0% (47/50) and 78.0% (39/ 50) respectively; in FA, positive rates were 0, 6.7% (3/45) and 0 respectively; in BPL, positive rates were 7.0% (3/34), 2.1% (1/47) and 0, respectively. The differences between PTC and FA and between PTC and BPL were both statistically significant (P = 0. 000). The sensitivity of Twist, HBME-1 and CK19 was 100%, 94.0% and 78.0%; the specifity was 96.4%, 95.7% and 100%; overall accurary was 97.7%, 95.1% and 91.9%, respectively.
CONCLUSIONSPositive rates of Twist is higher than the other markers in PTC. Immunohistochemical staining of Twist has important significance in the differential diagnosis of thyroid lesions. Twist immunohistochemistry maybe helpful in diagnosis and differential diagnosis of PTC.
