DNA Double-Strand Breaks, Potential Targets for HBV Integration
10.1007/s11596-010-0341-8
- Author:
HU XIAOWEN
1
,
2
;
LIN JUSHENG
;
XIE QIONGHUI
;
REN JINGHUA
;
CHANG YING
;
WU WENJIE
;
XIA YUJIA
Author Information
1. Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2. Department of Gastroenterology, Jinan Central Hospital, Shandong University, Jinan 250013, China
- Keywords:
DNA double-strand breaks;
hepatitis B virus;
integration;
non-homologous end joining
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(3):265-270
- CountryChina
- Language:Chinese
-
Abstract:
Hepatitis B virus (HBV)-induced hepatocellular carcinoma (HCC) is one of the most frequently occurring cancers. Hepadnaviral DNA integrations are considered to be essential agents which can promote the process of the hepatocarcinogenesis. More and more researches were designed to find the relationship of the two. In this study, we investigated whether HBV DNA integration occurred at sites of DNA double-strand breaks (DSBs), one of the most detrimental DNA damage. An 18-bp I-SceI homing endonuclease recognition site was introduced into the DNA of HepG2 cell line by stable DNA transfection, then cells were incubated in patients' serum with high HBV DNA copies and at the same time, DSBs were induced by transient expression of I-SceI after transfection of an I-SceI expression vector. By using nest PCR, the viral DNA was detected at the sites of the break. It appeared that integration occurred between part of HBV x gene and the I-Scel induced breaks. The results suggested that DSBs, as the DNA damages, may serve as potential targets for bepadnaviral DNA insertion and the integrants would lead to widespread host genome changes necessarily. It provided a new site to investigate the integration.
