Typing of 24 mtDNA SNPs in a Chinese Population Using SNaPshot Minisequencing
10.1007/s11596-010-0345-4
- Author:
HUANG DAIXIN
1
;
GUI CHENG
;
YI SHAOHUA
;
YANG QINGEN
;
YANG RONGZHI
;
MEI KUN
Author Information
1. Faculty of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
- Keywords:
mtDNA;
SNP;
SNaPshot minisequencing;
haplotype;
haplogroup
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(3):291-298
- CountryChina
- Language:Chinese
-
Abstract:
Three SNaPshot multiplex assays were developed to test 23 coding region single nucleo-tide polymorphisms (SNPs) and one control region SNP outside hypervariable regions (HVR) Ⅰ and Ⅱ, which was aimed at increasing the discrimination power of the mitochondrial DNA (mtDNA) typing in forensic casework, and confirming haplogroup assignments of mtDNA profiles in both hu-man population studies and medical research. The selected SNPs targeted the East Asian phylogeny. These multiplex assays were validated by comparing with the sequencing analysis of samples chosen randomly. The mtDNA variations of 100 unrelated individuals from the Wuhan population in China were examined and classified into 3 i haplotypes, and the haplotype diversity was estimated to be 0.952. The multiplex SNaPshot method is rapid and robust, and suitable for large-scale screening studies of mtDNA variability.
