A Possible Mechanism Linking Hyperglycemia and Reduced High-density Lipoprotein Cholesterol Levels in Diabetes
10.1007/s11596-010-0349-0
- Author:
GAO FENG
1
;
YAN TONG
;
ZHAO YAN
;
YIN FAN
;
HU CUINING
Author Information
1. Department of Endocrinology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China
- Keywords:
reverse cholesterol transport;
diabetes;
high-density lipoprotein cholesterol;
ATP-binding cassette transporter A1
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(3):318-321
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol(HDL-C).Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco's modified Eagle's medium(DMEM)containing glucose of various concentrations.The cells were divided into 3 groups in terms of different glucose concentrations in the cultures:Control group (5.6 mmol/L glucose),high glucose concentration groups(16.7 mmol/L and 30 mmol/L glucose).ATP-bindmg cassette transporter A1(ABCA1)mRNA expression in the macrophages was detected by semi-quantitative RT-PCR 24,48 and 72 h after glucose treatment.The results showed that ABCA1 mRNA expression in the 16.7 mmol/L glucose group was not significantly different from that in the control group at all testing time points(P＞0.05 for each).In the 30 mmol/L glucose group,macrophage ABCA1 mRNA expression was not changed significantly at 24 h(P=0.14),but was substantially decreased by 40.4% at 48 h(P=0.009)and by 48.1% at 72 h(P=0.015)as compared with that in the control group.It was concluded that ABCA1 is of vital importance for HDL-C biogenesis.High glucose may hamper HDL-C biogenesis by decreasing ABCA1 expression,which contributes to low HDL-C level in diabetes.
