Rabbit Annulus Fibrosus Cell Apoptosis Induced by Mechanical Overload via a Mitochondrial Apoptotic Pathway
10.1007/s11596-010-0361-4
- Author:
XIE MAO
1
;
YANG SHUHUA
;
Win Latt HEIN
;
XIONG LIMING
;
HUANG JIJUN
;
ZHOU JIANGUO
Author Information
1. Department of Orthopaedics,Union Hospital Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China
- Keywords:
pressure;
annulus fibrosus;
apoptosis;
mitochondrial membrane potential;
caspase
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(3):379-384
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the apoptotic pathway of rabbit annulus fibrosus(AF)cells induced by mechanical overload,an experimental air-pressure model was established in this study to pressurize the rabbit AF cells in vitro.Cells were randomly divided into five groups in which the cells were exposed to a continuous pressure of 1.1 MPa for different lengths of time(0,5,12,24 and 36 h).The cell proliferation and apoptosis were detected by cell counting kit-8(CCK-8)assay and flow cytometry; thealterations in mitochondrial membrane potential were measured by fluorescence microscopy and fluorescence spectrophotometer; the activities of caspase-8 and 9 were determined by spectrophotometry.The results showed that after the cells were subjected to the pressure for 24 or 36 h,the cell proliferation was inhibited; the ratio of cell apoptosis was increased; the mitochondrial membrane potential was decreased;the activity of caspase-9 was enhanced; no activity changes were observed in caspase-8.The results suggested that treatment with a pressure of 1.1 MPa for more than 24 h can lead to the proliferation inhibition and the apoptosis of rabbit AF cells in vitro,and the mitochondrial-dependent pathway is implicated in the pressure-induced AF cell apoptosis.
