Effect of Different Concentrations of Neogenin on Proliferation,Apoptosis and Related Proliferative Factors in Human Trophoblasts
10.1007/s11596-010-0457-x
- Author:
ZHONG SHAOPING
1
,
2
;
ZOU LI
;
ZHAO YIN
;
HU BIN
;
XIE HAN
Author Information
1. Department of Obstetrics and Gynecology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology Wuhan 430022,China
2. Department of Obstetrics,Women and Children Hospital,Jiaxing University,Jiaxing 314000,China
- Keywords:
neogenin;
trophoblast;
proliferation;
apoptosis;
proliferative factors
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(4):500-504
- CountryChina
- Language:Chinese
-
Abstract:
The underlying effect of different concentrations of neogenin on proliferation,apoptosis and the related proliferative factors in human trophoblasts was explored in order to understand the function of neogenin during placentation.TEV-1 cell line was cultured and the expression of netrin-1was detected by using indirect cellular immunofluorescence.Exponentially growing TEV-1 cells were treated by different concentrations of neogenin(0,1,5,10,50 ng/mL)for 24 h.Cell viability was measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)assay.TEV-1 cell apoptosis was assessed by flow cytometry(FCM).The expression of netrin-1 mRNA and protein in TEV-1 cells was examined by using real-time PCR and Western blot,respectively.It was found that immunoreactivity for netrin-1 was observed in cytoplasm of the trophoblasts.Immediately after treatment with different concentrations of neogenin for 24 h,the netrin-1 expression began to increase.Real-time PCR revealed that the expression level of netrin-1 mRNA was 37.59±10.25 times higher than control group when TEV-1 cells were exposed to 50 ng/mL neogenin(P<0.01),and the same tendency was seen by using Western blot.MTT results showed that proliferation of TEV-1 cells was independent of neogenin.Meanwhile,apoptosis was significantly increased to(22.15±6.15)% at50 ng/mL neogenin and(6.55±0.25)% without neogenin(P<0.01).It is suggested that neogenin regulates proliferation and apoptosis of TEV-1 cells.And it can enhance the ability of TEV-1 ceils to express netrin-1 in a dose-dependent manner.Neogenin may play an important biological role in the normal human pregnancy and contribute to the physiological pregnancy process.
