Induction of Cardiomyocyte Apoptosis by Anti-Cardiac Myosin Heavy Chain Antibodies in Patients with Acute Myocardial Infarction
10.1007/s11596-010-0546-x
- Author:
LIU KUN
1
;
SHAO LIANG
;
WANG LI
;
DING YANPING
;
SU GUANHUA
;
WANG JUE
;
LIAO YUHUA
;
WANG ZHAOHUI
Author Information
1. Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
- Keywords:
autoantibody;
cardiac myosin heavy chain;
apoptosis;
p53;
Bcl-2;
calcium
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2010;30(5):582-588
- CountryChina
- Language:Chinese
-
Abstract:
Autoimmune is involved in the pathogenesis of ventricular remodeling in acute myocardial infarction (AMI). In the present study, we investigated the effect of anti-cardiac myosin heavy chain antibodies (AMHCA) from patients with AMI on rat cardiomyocyte apoptosis. Cardiomyocyte apoptosis was observed and measured by DNA end labeling and Annexin- V/PI double-staining assay.The expression of apoptosis related p53 and Bcl-2 protein and the second messenger calcium were detected respectively by Western blotting, patch clamp and confocal calcium imaging. The results showed that AMHCA was able to induce cardiomyocyte apoptosis in a dose dependent manner.Apoptosis-accelerating nucleoprotein p53 was up-regulated, while apoptosis-inhibiting cytoplasmic protein Bcl-2 was down-regulated. In parallel, cytoplasmic calcium concentration was elevated.There was no effect on L-type calcium currents. It is concluded that AMHCA in patients with AMI as a novel triggering factor can induce cardiomyocyte apoptosis, which contributes to ventricular remodeling.
