Effect of G-CSF on induction of ENA-78 and IL-8 in the patients with malignant lymphoma.
10.7534/j.issn.1009-2137.2014.02.015
- Author:
Wan-Hong ZHAO
1
;
Shan MENG
1
;
Hideto TAMURA
2
;
Asaka KOND
3
;
Kiyoyuki OGATA
3
;
Kazuo DAN
3
Author Information
1. Department of Hematology, The Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 71004, Shaanxi Province, China.
2. Division of Hematology, Department of Medicine, Nippon Medical School, Tokyo113-8603, Japan. E-mail: tam@nms.ac.jp.
3. Division of Hematology, Department of Medicine, Nippon Medical School, Tokyo113-8603, Japan.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Antineoplastic Combined Chemotherapy Protocols;
adverse effects;
Chemokine CXCL5;
metabolism;
Female;
Granulocyte-Macrophage Colony-Stimulating Factor;
pharmacology;
Humans;
Interleukin-8;
metabolism;
Lymphoma;
metabolism;
Male;
Middle Aged;
Neutropenia;
chemically induced;
metabolism;
Neutrophils;
drug effects;
metabolism;
RNA, Messenger;
genetics
- From:
Journal of Experimental Hematology
2014;22(2):344-348
- CountryChina
- Language:English
-
Abstract:
Granulocyte colony stimulating factor (G-CSF) restores neutrophil count in patients with chemotherapy-induced neutropenia. G-CSF can also induce production of epithelial neutrophil activating protein-78 (ENA-78) and interleukin-8 (IL-8), chemotactic factors from neutrophils in vitro. This study was purposed to investigate whether this effect is also observed in vivo. 10 lymphoma patients were selected who received chemotherapy and G-CSF (nartograstim) administration. Blood was obtained before chemotherapy [Time Point 1 (TP1)], at neutropenic phase before G-CSF administration (TP2), and at neutrophil recovery phase after G-CSF (TP3). ENA-78 and IL-8 mRNA in neutrophils were quantified by real-time PCR. Phagocytosis and reactive oxygen species (ROS) generation were examined by flow cytometry. The results showed that ENA-78 and IL-8 mRNA expression at TP2 increased in 5 and 8 patients, respectively. The ENA-78 mRNA expression at TP3 was increased in 3 and decreased in 6 patients, and IL-8 mRNA expression at TP3 decreased in 7 patients. G-CSF did not affect phagocytosis and normalized ROS generation in all of the patient. It is concluded that increase of ENA-78 and IL-8 expression in neutrophils is common in chemotherapy-induced neutropenic patients. G-CSF administration does not significantly increase ENA-78 and IL-8 expression.
