Detection, diagnosis and analysis of the first case of neonatal alloimmune thrombocytopenia purpura associated with anti-HPA-5b in China.
10.7534/j.issn.1009-2137.2014.02.024
- Author:
Yan ZHOU
1
;
Zhou-Lin ZHONG
1
;
Li-Lan LI
1
;
Wei-Dong SHEN
1
;
Guo-Guang WU
2
Author Information
1. Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China.
2. Nanning Institute of Transfusion Medicine, Nanning Blood Center, Nanning 530007, Guangxi Zhuang Autonomous Region, China. E-mail: guangwu@szonline.net.
- Publication Type:Case Reports
- MeSH:
Antigens, Human Platelet;
genetics;
China;
Female;
Genotype;
Humans;
Infant, Newborn;
Purpura, Thrombocytopenic, Idiopathic;
diagnosis;
genetics;
Thrombocytopenia, Neonatal Alloimmune;
diagnosis;
genetics
- From:
Journal of Experimental Hematology
2014;22(2):399-402
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the detection and diagnosis of the neonatal alloimmune thrombocytopenia purpura (NAITP) caused by anti-HPA-5b antibody. The platelet count and clinical manifestation in the newborn were examined. The HPA-1-21bw genotypes of the newborn and her parents were detected by multiple-PCR and DNA sequencing. The HPA-specific antibody in the sera of newborn and her mother were detected and identified by flow cytometry (FCM) and monoclonal antibody-specific immobilization of platelet antigens (MAIPA). The results indicated that the clinical manifestations of the newborn were lighter. The HPA genotyping showed that the genotype of the newborn was HPA-5ab, while that of her mother and father were HPA-5aa and HPA-5ab, respectively. The antibody against the platelet of newborn's father existed in the newborn's mother sera. The HPA antibody of the mother was identified as anti-HPA-5b. It is concluded that the newborn with neonatal alloimmune thrombocytopenia purpura was caused by the antibody against HPA-5b.
