Significance of regulatory B cells in nosogenesis of immune thrombocytopenia.
10.7534/j.issn.1009-2137.2014.02.025
- Author:
Xin LI
1
;
Fang WANG
1
;
Kai Yang DING
2
;
Lan DAI
3
Author Information
1. Department of Hematology,Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China.
2. Department of Hematology,Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China. E-mail:dingkaiy@126.com.
3. Jiangsu Institute of Hematology, The First Affiliated Hospital of Suzhou University, Suzhou 215000, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Adult;
B-Lymphocytes, Regulatory;
immunology;
Case-Control Studies;
Female;
Flow Cytometry;
Humans;
Interleukin-10;
blood;
Male;
Purpura, Thrombocytopenic, Idiopathic;
blood;
immunology;
Transforming Growth Factor beta1;
blood
- From:
Journal of Experimental Hematology
2014;22(2):403-406
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the role of regulatory B cells (Breg) in pathogenesis of immune thrombocytopenia (ITP) and its clinical significance. A total of 35 ITP patients and 20 normal controls were enrolled in this study. The expression of CD19(+)CD24(hi)CD38(hi) B cells was detected by flow cytometry and the expression of IL-10 mRNA and TGF-β1 mRNA was assayed by RT-PCR. The results indicated that the expression level of CD19(+)CD24(hi)CD38(hi) B cells in peripheral blood of newly diagnosed ITP patients was obviously lower than that in normal controls (P < 0.05); the expression level of CD19(+)CD24(hi)CD38(hi) B cells in ITP patients with increased platelet count after treatment was higher than that before treatment (P < 0.05); the expression level of IL-10 mRNA in newly diagnosed ITP patients was significantly lower than that the in normal controls (P < 0.05), the expression level of TGF-β1 mRNA in newly diagnosed ITP patients increases as compared with normal controls (P < 0.05), after treatment with DXM the expression of IL-10 mRNA was enhanced, the expression of TGF-β1 mRNA was reduced as compared with expression level before treatment (P < 0.05). It is concluded that the Breg cells may play an important role in the pathogenesis of ITP via humoral immunity and its regulation of T lymphocytes.
