Effect of GW003 on the granulocyte macrophage colony formation ability of bone marrow cells in vitro.
10.7534/j.issn.1009-2137.2014.02.038
- Author:
Hong-Ling OU
1
;
Shuang XING
2
;
Ming LI
3
;
Guo-Lin XIONG
2
;
Feng-Hua CHEN
1
;
Xiao-Ning LI
1
;
Xin-Ru WANG
4
Author Information
1. Department of Clinical Laboratorial Examiaction, General Hospital of the Chinese Second Artillery, Beijing 10088, China.
2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
3. Radiation Medicine and Public Health College, Suzhou University, Suzhou 215123, Jiangsu Province, China.
4. Department of Clinical Laboratorial Examiaction, General Hospital of the Chinese Second Artillery, Beijing 10088, China. E-mail:wangxinru@126.com.
- Publication Type:Journal Article
- MeSH:
Adult;
Animals;
Bone Marrow Cells;
drug effects;
Cell Line, Tumor;
Colony-Forming Units Assay;
Female;
Granulocyte Colony-Stimulating Factor;
pharmacology;
Granulocyte-Macrophage Progenitor Cells;
cytology;
drug effects;
Granulocytes;
drug effects;
Humans;
Macaca mulatta
- From:
Journal of Experimental Hematology
2014;22(2):475-478
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the effect of GW003 on the ability of granulocyte colony forming in vitro of bone marrow cells. The bone marrow samples was collected from normal rhesus, the patients with leukemia in stages of remission and chemotherapy respectively, and the nucleated cells were separated and cultured for 12 days after addition of different concentrations of GW003 or rhG-CSF, or G-CSF mutant. Then the amount of colony-forming unit-granulocyte-macrophage was counted. The results indicated that GW003 could enhance the ability of bone marrow nucleated cells of rhesus to forming CFU-GM in vitro, and its effect was much better than that of rhG-CSF or G-CSF mutant at the same concentration(®). The GW003 showed dose-response relationship to CFU-GM level (r = R(2) = 0.965, P = 0.003, in a certain concentration), the GW003 also could enhance CFU-GM formation of marrow nucleated cells in leukemic patients, especially for patients receiving chemotherapy. The GW003 could relieve the marrow suppression caused by chemotherapy significantly. It is concluded that the GW003 can significantly improve the ability of bone marrow cells to form granulocyte colony in vitro as well as effectively alleviate bone marrow suppression.
