Identification of a novel HLA allele, HLA-DRB1*03:80, by sequencing-based typing.

Author: Xiang-Min NIE ¹ ; Yi ZHANG ¹ ; Yun-Long ZHUANG ¹ ; Yong-Hong SONG ¹ ; Wen-Ben QIAO ¹ ; Yan LIU ¹ ; Chuan-Fu ZHU ²
Author Information

1. HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China.
2. HLA Typing Laboratory of China Marrow Donor Program, Blood Center of Shandong Province, Jinan 250014, Shandong Province, China. E-mail:zhuchuanfu2008@tom.com.
Publication Type:Journal Article
MeSH: Alleles; Asian Continental Ancestry Group; genetics; HLA-DRB1 Chains; genetics; Humans; Male; Sequence Analysis, DNA
From: Journal of Experimental Hematology 2014;22(2):509-512
CountryChina
Language:Chinese
Abstract: This study was aimed to identify a novel HLA-DRB1 allele from a Chinese potential hemopoietic stem cell donor of Northeast China. A rare HLA-DRB1 allele was initially detected by Luminex PCR-SSO typing, then the sample was sequenced by sequence-based typing (SBT) and the alignments of sample's alleles was identified by single allele-specific sequencing strategy. The results revealed the existence of a new allele which differs from the closest matching allele DRB1*03:06 by a single nucleotide substitution at position 239, where C→G in exon 2, resulting in an amino acid exchange from Thr to Arg at codon 51. It is concluded that a novel allele has been confirmed and its name DRB1*03:80 is officially assigned by the WHO Nomenclature Committee in February 2012.