IFN-γ stimulation enhances immunosuppressive capability of human umbilical cord mesenchymal stem cells.
10.7534/j.issn.1009-2137.2014.03.006
- Author:
Xiao-Dun LIU
1
;
Dong LIU
1
;
Chuan-Bao ZANG
1
;
Hai-Yan ZHANG
1
;
Yi MING
1
;
Ling-Long CHI
1
;
Bai-Jun SHEN
1
;
Dong LI
2
;
Author Information
1. Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China.
2. Department of Resench and Development, Cord Blood Stem Cell Bank of Shandong Province, Jinan 250000, Shandong Province, China
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Humans;
Immunosuppression;
Interferon-gamma;
pharmacology;
Mesenchymal Stromal Cells;
cytology;
drug effects;
immunology;
Umbilical Cord;
cytology
- From:
Journal of Experimental Hematology
2014;22(3):605-611
- CountryChina
- Language:Chinese
-
Abstract:
This study was objective to explore the effect of IFN-γ on immunosuppressive capability of mesenchymal stem cells (MSC) derived from umbilical cord. The immunomodulating capability of MSC was changed by stimulating cell surface receptors like Toll-like receptors (TLR). The inhibition of T-lymphocyte proliferation by MSC was tested via cell co-cultures. Further RT-PCR and ELISA were performed to examine the expression changes in gene and protein level. The results showed that the IFN-γ could promote the immunosuppressive effect of umbilical cord derived MSC. IFN-γ-stimulated MSC could suppress the proliferation of T cells more effectively. IFN-γ stimulation up-regulated the expression of immunosuppressive genes like IDO1, COX2, HLA-G, and soluble suppressive proteins such as HLA-G, KYN, IL10, PGE2 of MSC. And the immuno suppression capability of IFN-γ-stimulated MSC was 2-7 folds higher than control in MSC and lymphocyte co-culture tests. It is concluded that IFN-γ can effectively enhance the immunosuppressive capability of MSC.
