Expression of P2X family receptors in peritoneal macrophages of mouse with acute T lymphoblastic leukemia.
10.7534/j.issn.1009-2137.2014.03.009
- Author:
Sha-Yan CHEN
1
;
Wen-Li FENG
1
;
Xiao YANG
2
;
Jin-Feng LIAO
2
;
Li-Na WANG
2
;
Yong-Min LIN
2
;
Qian REN
2
;
Guo-Guang ZHENG
3
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology,Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
3. State Key Laboratory of Experimental Hematology,Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. E-mail: zhenggg@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Cytokines;
metabolism;
Disease Models, Animal;
Macrophages, Peritoneal;
metabolism;
Mice;
Mice, Inbred C57BL;
Precursor Cell Lymphoblastic Leukemia-Lymphoma;
metabolism;
Receptor, Notch1;
metabolism;
Receptors, Purinergic P2X;
metabolism;
Signal Transduction
- From:
Journal of Experimental Hematology
2014;22(3):623-628
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at exploring the expression pattern of P2X family receptors (P2XR) in peritoneal macrophages and their relationship with the activation states of macrophages in Notch1-induced mouse T-ALL model. After establishment of the leukemia model, F4/80(+) peritoneal macrophages, F4/80(+)CD206(+) M2-like and F4/80(+)CD206(-) M1-like peritoneal macrophages were sorted by flow cytometry based on F4/80 and CD206 surface markers. The expression of P2XR in each cell population was detected by real time RT-PCR. The results showed that macrophages,M1-like and M2-like macrophages moderately expressed P2XR except for P2X5R. The expression of P2XR varied with the development of leukemia. The expression of P2X1R and P2X7R in peritoneal macrophages increased steadily; the expression of P2X2R and P2X3R decreased at late stage of leukemia;the expression of P2X4R slightly decreased at intermediate stage;the expression of P2X6R kept unchanged. At intermediate stage of leukemia, the expression of P2XR in M1-like and M2-like peritoneal macrophages varied. M1-like macrophages expressed higher level of P2X1R than M2-like macrophages, whereas M2-like macrophages expressed higher level of P2X7R than M1-like macrophages, which suggested that the expression of P2XR were related to the activation states. It is concluded that the expression of P2XR in peritoneal macrophages from leukemia mice is related to the progression of leukemia and the activation states of macrophages, which lay a foundation for further studying the role of macrophages in the development of leukemia.
