Effect of CIAPIN1 gene on proliferation of K562 cells.
10.7534/j.issn.1009-2137.2014.03.018
- Author:
Ya-Ni LIN
1
;
Jian WANG
1
;
Qing-Hua LI
1
;
Hua XU
1
;
Hai-Rui ZHANG
1
;
Tian-Xiang PANG
2
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
2. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. E-mail: pang@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Cell Proliferation;
Genetic Vectors;
Humans;
Intracellular Signaling Peptides and Proteins;
genetics;
K562 Cells;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive;
genetics;
RNA, Small Interfering;
Transfection
- From:
Journal of Experimental Hematology
2014;22(3):671-674
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the effect of CIAPIN1 gene on the proliferation of chronic myeloid leukemia (CML) cell line K562. The shRNA eukaryotic expression vector targeting CIAPIN1 gene was constructed and transfected into K562 cells. The inhibitory efficiency on K562 cells was detected by real-time PCR and Western blot; the proliferative activity of K562 cells was detected by MTT assay; the number and size of colonies were assessed by using colony-forming test; the tumorigenic potential was tested in vivo by using nude mice. The results indicated that as compared with control group, the CIAPIN1 gene expression statistically decreased; the proliferative activity of K562 cells in interference group was distinctly weakened; the number and size of colonies were significantly reduced; the tumorigenic potential was also lowered in vivo. It is concluded that inhibition of CIAPIN1 expression can inhibit K562 cell proliferation in vitro and in vivo.
