Inducing-apoptosis effect of brucine on human monocytic leukemia cell line THP-1 and its mechanism.
10.7534/j.issn.1009-2137.2014.03.020
- Author:
Fei XIN
1
;
Wu WEI
2
;
Ai-Fang JI
3
;
Xu-Liang SHEN
3
;
Guo-Xiang ZHANG
3
;
Mei-Xiang ZHANG
3
;
Xian-Xian LI
1
;
Hai-Yan ZHANG
1
Author Information
1. First clinicial Department, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China.
2. Department of Hematology, Peace Hospital Affiliated to Changzhi Medical college, Changzhi 046000, Shanxi Province, China. E-mail: weiwu88@sohu.com.
3. Department of Hematology, Peace Hospital Affiliated to Changzhi Medical college, Changzhi 046000, Shanxi Province, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Cycle;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Strychnine;
analogs & derivatives;
pharmacology;
bcl-2-Associated X Protein;
metabolism
- From:
Journal of Experimental Hematology
2014;22(3):681-686
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the inducing-apoptosis effect of brucine on human monocytic leukemia cell line THP-1 cells and its possible mechanism. The inhibition effect of brucine on growth of THP-1 cells was measured by CCK-8 method. Morphological changes of THP-1 cells treated with brucine was detected by acridine orange/ethidium bromide (AO/EB)double staining. Annexin-V/PI double labeling method was used to assay the apoptosis rate of THP-1 cells. The effect of brucine on THP-1 cell cycle distribution was detected by PI single staining. RT-PCR was used to detect the expression of BCL-2 and BAX. The results showed that the brucine could inhibit the THP-1 cell growth in concentration and time-dependent manners at the range of 50 to 400 µg/ml. The cells stained with AO/EB revealed that the brucine induced the nuclear chromatin condensation. After the THP-1 cells were treated with brucine of 400µg/ml for 48 hours, most nucleic were stained as orange-red, and condensed, displaying the late apoptotic cell morphology. Annexin-V/PI detection showed that brucine could induce apoptosis of THP-1 cells in a concentration-dependent manner. Compared with the control group, more cells in brucine-treated group were arrested at G0/G1 phase in a concentration-dependent manner. RT-PCR detection revealed that the expression of BCL-2 was down-regulated strikingly and BAX was up-regulated. It is concluded that brucine can efficiently inhibit cell growth and block THP-1 cells in G0/G1 phase. The mechanism of THP-1 cell apoptosis induced by brucine may be related to the inhibition of BCL-2 and activation of BAX.
