Role of BCL-2, caspase-3 and NF-κB in astragaloside inducing apoptosis of human NB4 cells.
10.7534/j.issn.1009-2137.2014.03.024
- Author:
Jing NI
1
;
Xi XIE
1
;
Jing XIE
1
;
Xue-Ying HU
1
;
Zhen-Qi HUANG
1
;
Rui-Xiang XIA
2
Author Information
1. Department of Homatology, The Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China.
2. Department of Homatology, The Affiliated Hospital, Anhui Medical University, Hefei 230022, Anhui Province, China. E-mail: xrx2041@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
NF-kappa B;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Saponins;
pharmacology
- From:
Journal of Experimental Hematology
2014;22(3):703-706
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the apoptosis-inducing effect of astragalosides on acute promyelocytic leukemia(APL) cell line NB4 and its mechanism. NB4 cells were treated with different concentrations (200, 300, 400 µg /ml) of astragalosides for 48 h. The cell proliferation was assayed by using CCK-8 method; the cell apoptosis was analyzed by flow cytometry with Annexin V-FITE/PI double staining. The mRNA expression of BCL-2 and the relative activity of BCL-2, NF-κB and caspase-3 were detected by RT-PCR and Western blot, respectively. The results showed that after treated with astragalosides for 48 h, astragalosides inhibited NB4 cell proliferation in concentration-dependent way, the apoptosis rate of NB4 cells gradually elevated from 4.69% to 40.85% with the increasing of astragalosides concentration. Simultaneously, the mRNA expression of BCL-2 was down-regulated, Western blot analysis showed that the protein expression levels of BCL-2 and NF-κB decreased after astragalosides treatment, while caspase-3 protein expression level increased. It is concluded that the molecular mechanism of the astragalosides-induced apoptosis in NB4 cells may be associated with down-regulation of the expression of BCL-2 and NF-κB, finally the relative activity of caspase-3 activated.
