Identification and analysis of a mouse gene homologous to human hepatitis B virus pre-S1 protein-binding protein using the bioinformatics method.
- Author:
Jun CHENG
1
;
Jing DONG
;
Jian-Jun WANG
;
Jian ZHANG
;
Dong JI
;
Yan-Wei ZHONG
;
Yan LIU
;
Lin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Base Sequence; Carrier Proteins; genetics; Cloning, Molecular; Computational Biology; DNA, Complementary; genetics; Databases, Nucleic Acid; Hepatitis B Surface Antigens; genetics; Hepatitis B virus; genetics; Humans; Mice; Molecular Sequence Data; Protein Precursors; genetics; Sequence Alignment; Sequence Homology, Nucleic Acid
- From: Chinese Journal of Hepatology 2005;13(4):241-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo clone and identify the mouse gene homologous to human hepatitis B virus (HBV) pre-S1 protein-binding protein (PS1BP).
METHODSThe human PS1BP cDNA sequence was used as the reference sequence to search homologous mouse cDNA sequence from GenBank established by National Center for Biotechnology (NCBI), National Institute of Health (NIH), for its homologous cDNA sequences of mouse by BLASTn tool. The characteristics of mouse PS1BP protein primary structure were predicted by online software. Finally the genomic DNA structure of mouse PS1BP was deduced and compared.
RESULTSThe mouse PS1BP was identified and consisted of 1455 nt, coding a protein of 484 aa. The identity of human and mouse PS1BP protein is 84.92% (411/484). The genomic DNA of mouse PS1BP consisted of 3 exons and 2 introns.
CONCLUSIONThe identification and characterization of mouse PS1BP cDNA and genomic DNA pave a way for further study of their structures and functions.
