Two-dimensional gel electrophoresis of subcellular fractions of hepatoma cells.
- Author:
Xing LI
1
;
Wei PAN
;
Feng QIU
;
Kun-Lun TIAN
;
Zong-Yin QIU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; chemistry; pathology; Electrophoresis, Gel, Two-Dimensional; Humans; Liver Neoplasms; chemistry; pathology; Neoplasm Proteins; analysis; Proteome; Tumor Cells, Cultured
- From: Chinese Journal of Hepatology 2005;13(4):271-273
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo seek a better profiling of proteins of hepatoma cells.
METHODSThe homogenate of hepatoma cells QGY-7703 was fractionated into four parts by differential centrifugation: the nuclei, the pellet by 20,000 x g, the pellet by 100,000 x g and the cytosolic supernatant. The four fractions were submitted to two-dimensional gel electrophoresis and their electrophoretic patterns were analyzed.
RESULTSIn comparison with the protein pattern of hepatoma cells not fractionated, the patterns of the four fractions display many more protein spots, and a large number of proteins present in the nuclei and cytosolic supernatant were not shown in the not-fractionated samples.
CONCLUSIONPreparation of subcellular fractions before electrophoretic procedures proves to be very useful; not only can it improve the results of two-dimensional gel electrophoresis, but also can lead to research into the subcellular level.
