A study of the association of iNOS and eNOS gene polymorphism with portal hypertension in liver cirrhosis.
- Author:
Yuan-qiao CHENG
1
;
Ju-sheng LIN
;
Wen-qi WANG
;
Ping XIONG
;
Xiao-dan JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Female; Hepatitis B, Chronic; complications; Humans; Hypertension, Portal; etiology; genetics; Liver Cirrhosis; complications; genetics; Male; Middle Aged; Nitric Oxide Synthase Type II; genetics; Nitric Oxide Synthase Type III; genetics; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Promoter Regions, Genetic; genetics
- From: Chinese Journal of Hepatology 2005;13(5):366-369
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study whether polymorphism of iNOS and eNOS genes is associated with portal hypertension in liver cirrhosis.
METHODSA case control study of 106 patients with liver cirrhosis due to HBV was performed in comparison with 108 controls using PCR-RFLP to detect iNOS promoter -969G --> C and eNOS exon7 894G --> T polymorphism.
RESULTSThe frequency of the C allele and GC genotype in the iNOS promoter -969G --> C was significantly higher in the portal hypertension group than in the control group. (P < 0.05). The frequency of the T allele and GT genotype in eNOS exon7 894G --> T was dramatically higher in the portal hypertension group than in the control group (P < 0.05). Multivariate logistic regression analysis revealed that iNOS polymorphism in iNOS promoter -969G --> C and eNOS exon7 894G --> T was an independent new risk factor for portal hypertension. We discovered that iNOS promoter -969G --> C led to an increase in its functional activity.
CONCLUSIONSThe polymorphism of iNOS promoter -969G --> C and eNOS exon7 894G --> T is associated with portal hypertension of liver cirrhosis, which is a new independent risk factor found related to the occurrence of portal hypertension. The polymorphism of iNOS promoter -969G --> C results in functional activity increase of the iNOS promoter.
