Sequence analysis and identification of two novel HLA alleles HLA-B*9536 and HLA-B*4612.
- Author:
Yanmin HE
1
;
Wei ZHANG
;
Junjun HE
;
Wei WANG
;
Zhedong HAN
;
Nanying CHEN
;
Faming ZHU
;
Lixing YAN
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Asian Continental Ancestry Group; genetics; Base Sequence; HLA-B Antigens; genetics; HLA-B15 Antigen; Humans; Male; Molecular Sequence Data
- From: Chinese Journal of Medical Genetics 2009;26(6):678-680
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify two novel HLA alleles HLA-B*9536 and B*4612, in an individual.
METHODSDNA was extracted from whole blood by Invitrogen DNA extraction kit. The amplification for HLA-B exons 2-4 of the proband was performed separately with allele group specific primers and the PCR products were directly sequenced for exons 2-4 in both direction.
RESULTSThere were two novel HLA-B alleles in the proband. The sequences of the two alleles have been submitted to GenBank (EU081878 and EU081879). The two alleles have been officially named as B*9536 and B*4612 by the WHO Nomenclature Committee. The sequence of exons 2-4 of HLA-B*9536 showed one nucleotide difference in exon 3 at position 544 (G to A) comparing with the closest allele B*1505, which resulted in an amino acid change from Ala to Thr at codon 158. In the HLA-B*4612 allele, there was one nucleotide change in exon 3 at position 363 (G to A), when compared to the closest allele B*4601, which lead to an amino acid change from Arg to Ser at codon 97.
CONCLUSIONTwo novel HLA-B alleles were identified in one individual and have been officially named by the WHO Nomenclature Committee.
