Identification of a novel HLA allele HLA-B*9526.
- Author:
Yang CHEN
1
;
Xianzhi LIU
;
Xiaofeng LI
;
Xu ZHANG
;
Kunlian ZHANG
;
Jianping LI
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Amino Acid Sequence; Base Sequence; China; Cloning, Molecular; Genotype; HLA-B Antigens; genetics; HLA-B15 Antigen; Humans; Male; Molecular Sequence Data; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2009;26(6):705-708
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA novel human leukocyte antigen-B (HLA-B) allele, B*9526, was identified and analyzed by sequencing-based method in a Chinese donor.
METHODSHLA typing was performed by PCR-sequence-specific oligonucleotide (SSO). Molecular cloning and DNA sequencing were used to identify the sequence of the potential novel allele and the difference between this new allele and other known alleles were analyzed.
RESULTSHLA genotyping of one sample gave different results. The sequencing result showed HLA-B alleles of the proband as B*5403 and a novel allele. The nucleotide sequence of the novel allele was different from all known B alleles and harbored one nucleotide change from the closest matching allele B*1507 at nucleotide 425 (A to G) in exon 3, resulting in an amino acid change from Y (TAC) to C (TGC) at codon 142.
CONCLUSIONA novel HLA allele was identified and officially designated as HLA-B*9526 by WHO Nomenclature Committee for Factors of the HLA System.
