Establishment of immortalized cell lines and genetic stability evaluation for a family with spinocerebellar ataxia type 2.
- Author:
Ke-qin LIN
1
;
Hao SUN
;
Chang-jun ZHANG
;
Yu-fen TAO
;
Wen YI
;
Xiao-qin HUANG
;
Li SHI
;
Jia-you CHU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Asian Continental Ancestry Group; genetics; Cell Line, Transformed; Cell Transformation, Viral; Female; Herpesvirus 4, Human; physiology; Humans; Karyotyping; Male; Microsatellite Repeats; Pedigree; Spinocerebellar Ataxias; genetics; Young Adult
- From: Chinese Journal of Medical Genetics 2009;26(4):374-378
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEImmortalized cell lines of spinocerebellar ataxia type 2 (SCA2) with Parkinson disease symptoms were established in order to provide experimental material for future study.
METHODSThe immortalized cell lines were constructed by using Epstein Barr virus and cyclosporine A. Microsatellite markers were detected to see whether there is any change between the cell lines and the original blood samples, and the genetic stability of the cell lines were evaluated.
RESULTSTwenty-five immortalized cell lines were established successfully from the family and the microsatellite markers were unchanged.
CONCLUSIONThe karyotypes of the immortal cell lines were normal and the cell lines were genetically stable.
