Application of multicolor primed in situ labeling in simultaneous identification of chromosomes 18, X and Y in uncultured amniocytes.
- Author:
Li DENG
1
;
Xian-ping DING
;
Yong NIE
;
Yu HU
;
Hong-jing REN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Amniotic Fluid; chemistry; cytology; Cells, Cultured; Chromosomes, Human, Pair 18; chemistry; genetics; Chromosomes, Human, X; chemistry; genetics; Chromosomes, Human, Y; chemistry; genetics; Female; Humans; In Situ Hybridization, Fluorescence; methods; Pregnancy; Prenatal Diagnosis; methods; Primed In Situ Labeling; methods; Young Adult
- From: Chinese Journal of Medical Genetics 2009;26(4):439-442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a multicolor primed in situ labeling (PRINS) protocol for chromosome detection in uncultured amniocytes.
METHODSChromosomes 18, X and Y in uncultured amniocytes were simultaneously detected by using the non-ddNTP-blocking multicolor PRINS procedure.
RESULTSWithin 7 h, the 3 chromosomes were simultaneously marked in the same uncultured amniocyte. The chromosome signals were successfully detected in 69 uncultured samples of amniotic fluid. The results were consistent with that obtained by chromosomes in cultured amniocytes.
CONCLUSIONThis multicolor protocol was high throughput, fast, simple, sensitive and reliable in diagnosing chromosome abnormalities in uncultured amniocytes.
