Study on the differentially expressed genes of steroid-resistant and steroid-sensitive nephrotic syndrome.
- Author:
Rong-yan CHEN
1
;
Li YU
;
Zhi-hong HAO
;
Li-na WANG
;
Yue-qiang WEN
Author Information
- Publication Type:Journal Article
- MeSH: Child; Gene Expression Profiling; Gene Expression Regulation; drug effects; Humans; Nephrotic Syndrome; drug therapy; genetics; Steroids; therapeutic use
- From: Chinese Journal of Medical Genetics 2009;26(4):446-451
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the genes differentially expressed among the steroid-resistant nephrotic syndrome (SRNS), steroid-sensitive nephrotic syndrome (SSNS) and normal children, and understand the molecular mechanism of SRNS.
METHODSAffymetrix microarray technology was used to obtain such a profile. The differentially expressed genes among these groups were identified based on signal-to-noise ratios by GCOS software; real-time PCR analysis was performed to confirm the microarray results.
RESULTSThere were 157 genes differentially expressed among these groups. The genes up-regulated both in SRNS and SSNS were involved primarily in ionic transportation, immuno-signal transduction and apoptosis. In particular, CLNS1A gene was down regulated in SRNS but up regulated in SSNS.
CONCLUSIONSeveral differentially expressed genes, such as CLNS1A and HLA-DRB4 were found to be closely related to the pathogenesis of SRNS and SSNS. This DNA microarray analysis has provided some important clue to the molecular mechanism of SRNS.
