A clinical and laboratory study on acute myeloid leukemia with t(6;9)(p23;q34).
- Author:
Yong WANG
1
;
Yongquan XUE
;
Suning CHEN
;
Yafang WU
;
Jinlan PAN
;
Jun ZHANG
;
Juan SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Antigens, CD; genetics; Antigens, CD34; genetics; Antigens, Differentiation, Myelomonocytic; genetics; CD13 Antigens; genetics; Chromosomes, Human, Pair 6; genetics; Chromosomes, Human, Pair 9; genetics; Female; Humans; Leukemia, Myeloid, Acute; genetics; Male; Middle Aged; Proto-Oncogene Proteins c-kit; genetics; Sialic Acid Binding Ig-like Lectin 3; Translocation, Genetic
- From: Chinese Journal of Medical Genetics 2010;27(1):34-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the clinical and laboratory features of 6 cases of acute myeloid leukemia (AML) with t(6;9)(p23;q34).
METHODSChromosome preparation of bone marrow cells was performed with regular method. R-banding by heating using Giemsa banding technique (RHG) was used for karyotype analysis. The immunoprofile was studied by flow cytometry (FCM) using a panel of monoclonal antibodies. Chromosome painting was performed by using whole chromosome paint probes for chromosomes 6 and 9 in all the 6 cases. The expression of fusion gene DEK/CAN and FLT3-ITD mutation were analyzed by reverse transcription-PCR(RT-PCR).
RESULTSThe t(6;9)(p23;q34) was found in all the 6 cases including 4 cases of M2 and 2 cases of M4. Blast cells were positive for CD13 and CD33 in 6 patients, for HLA-DR in 4 patients, for CD34 and CD117 in 3 cases, for CD38 or CD15 each in 1 case, respectively. A reciprocal translocation between chromosome 6 and 9 was confirmed by chromosome painting technique in the 6 cases. The DEK/CAN fusion gene was found in all the 6 cases, FLT3-ITD mutation was detected in three of them. Follow-up showed that 3 patients died with a survival time of 3 months, 5 months and 6 months, respectively. The other three obtained complete remission and are still alive.
CONCLUSIONThe t(6;9)(p23;q34) is a rare recurrent abnormity. AML with t(6;9)(p23;q34) has unique clinical and laboratory features and its prognosis is poor in most cases.
