The clinical and experimental analyses of a multiple myeloma patient with derivative der(Y;1).
- Author:
Tong WANG
1
;
Jing-ying QIU
;
Xiao-lan MA
;
Xiao-peng JIA
;
Yan-ping WANG
;
Hui-jun YU
;
Huan LI
;
Chun-rong TONG
Author Information
- Publication Type:Journal Article
- MeSH: Chromosome Aberrations; Chromosomes, Human, Y; genetics; Haplotypes; Humans; In Situ Hybridization, Fluorescence; Male; Middle Aged; Multiple Myeloma; genetics; therapy; Treatment Outcome
- From: Chinese Journal of Medical Genetics 2010;27(2):214-216
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the clinical significance of a rare chromosome abnormality der(Y)t(Y;1) in a patient with multiple myeloma (MM).
METHODSThe chromosome spread was prepared after 24 h culture of bone marrow. G-banding technique was used to analyze the karyotype. Fluorescence in situ hybridization (FISH) was performed to ascertain the origin of abnormal chromosome detected by conventional karyotypic analysis. Flow cytometry was used to detect the expression of the CD38/CD138/ZAP70. Immunoelectrophore was applied to identify the type of immunoglobulin.
RESULTSA complex pattern of chromosome rearrangement was observed: 92,XXYY[3]/49,X,der(Y)t(Y;1)(q12;q21),t(11;14)(q13;q32),+18,+20,+21[47]/49,X,idem,del(13q22),ace[1]/98,XX,der(Y)t(Y;1) x 2,+18,+18,+20,+20,+21,+21[10]/46,XY[19]. The result was confirmed by metaphase-FISH. The type of immunoglobulin was IgD with the level of 6.24g/L. The CD38/CD138 was positive but ZAP70 was negative.
CONCLUSIONStructural abnormality of chromosome Y is rare in blood malignancy. Most of them were described in myelodysplastic syndrome or myeloproliferative disorders. It is the first report of der(Y)t(Y;1) abnormality in multiple myeloma.
