Assessment of the two preparation techniques of sperm: swim \ufffd?up and discontinuous density gradient.
- Author:
Phuong Thi Thuy Dao
;
Binh Thi Nguyen
- Publication Type:Journal Article
- Keywords:
Sperm preparation;
swim up technique;
density gradient technique;
assisted reproductive technologies.
- MeSH:
Spermatozoa;
Centrifugation;
Density Gradient;
- From:Journal of Medical Research
2007;52(5):35-39
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: In Vietnam, the proportion infertile couples was about 7-10% of reproductive couples. Assisted reproductive technologies appeared and expanded rapidly. In 1970, sperm preparation techniques were found out, artificial insemination became widespread. Sperm preparation stage is very important in assisted reproductive techniques and increasingly improved. Objectives: To compare the efficiency of two sperm preparation techniques: swim \ufffd?up and discontinuous density gradient. Subjects and method: This was a cross-sectional described study included 30 normal semen samples were chosen based on WHO 1999 criteria. We examined sperm vitality, concentration, motility, morphology before and after applied these techniques. Results: Both procedures resulted in a significant higher percentage of vitality, motility, morphology compared with the original semen sample (p<0.01), but the concentration reduced approximately 5 times from whole semen to sediment when swim \ufffd?up was used and 3 times with density gradient, the difference was statically significant (p< 0.01). Conclusion: Normal semen should be prepared by these method. But in oligozoospermic man, to obtain a higher concentration of sperm, semen should be prepared by density gradient method.
