Quantification of GRAF gene expression in patients with acute myeloid leukemia using EvaGreen real time quantitative PCR.
- Author:
Zhen QIAN
1
;
Jiang LIN
;
Jun QIAN
;
Dong-ming YAO
;
Ya-li WANG
;
Lan-xiu HAN
;
Zhao-hui ZHU
;
Gao-fei XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; Female; GTPase-Activating Proteins; genetics; Humans; Leukemia, Myeloid, Acute; genetics; Male; Middle Aged; Polymerase Chain Reaction; methods; Young Adult
- From: Chinese Journal of Medical Genetics 2010;27(3):290-293
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo quantify the expression level of GRAF gene in acute myeloid leukemia (AML) and analyze its clinical significance.
METHODSThe EvaGreen real-time quantitative polymerase chain reaction (RQ-PCR) assay was established and performed to measure the GRAF gene transcripts in 71 cases with AML and 21 with nonmalignant hematological diseases. The clinical correlation of GRAF expression was analyzed.
RESULTSThe established EvaGreen RQ-PCR assay had good specificity, reproducibility and sensitivity. The GRAF expression level was significantly lower in AML (0.01%-169.75%, median 3.82%) than that in controls (14.49%-126.85%, median 56.04%) (P<0.05). There was no correlation between the level of GRAF transcript and the sex, age, hematologic parameters, FAB subtypes and karyotypic groups (P>0.05).
CONCLUSIONThe GRAF gene was down-regulated in AML, which might play a role in the leukemogenesis.
