Study on the relationship between partial deletions in the azzospermia factor C region of Y chromosome and male infertility.
- Author:
Xiao-su XIAO
1
;
Li-qing JIANG
;
Tian-yuan WANG
;
Yong-qiang WANG
;
Li-hua LU
;
Rong HE
Author Information
- Publication Type:Journal Article
- MeSH: Chromosomes, Human, Y; genetics; Genetic Loci; Humans; Infertility, Male; genetics; Male; Polymerase Chain Reaction; Seminal Plasma Proteins; genetics; Sequence Deletion; genetics
- From: Chinese Journal of Medical Genetics 2010;27(3):294-298
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between the partial deletions in the azzospermia factor(AZFc) region of Y chromosome and male infertility.
METHODSMultiplex PCR technology was performed to screen the partial deletions in the AZFc region in 158 azoospermia, 160 severe oligozoospermia and 135 oligozoospermia patients and 236 men with normal spermatogenesis.For samples with gr/gr, b2/b3 recombinogenic deletions, author applied RFLP method to identify which DAZ gene doublet deletion was involved.
RESULTSThe gr/gr and b2/b3 were two types of common deletions detected. There were significant differences in the b2/b3 deletion in patients with oligozoospermia and severe oligozoospermia compared to the controls (both P< 0.05). However, there was no difference for the gr/gr deletion between the patients and controls.
CONCLUSIONThe results suggested that the b2/b3 deletion might be a risk factor to spermatogenic impairment and might lead to male infertility.
