Study on the correlation of GCS and MDR1 genes in inducing multidrug resistance in human K562/A02 cell line.
- Author:
Guo-qing YANG
1
;
Ke-ming XIE
;
Yan LIU
;
Hui-jun MU
;
Wei-zhen QIAO
;
Bin ZHANG
;
Ping XIE
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; genetics; Cell Line, Tumor; Drug Resistance, Multiple; genetics; Drug Resistance, Neoplasm; genetics; Glucosyltransferases; genetics; Humans; RNA, Messenger; genetics; RNA, Small Interfering; genetics; physiology; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Medical Genetics 2010;27(3):299-304
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the correlation of glucosylceramide synthase (GCS) gene and multidrug resistance 1 (MDR1) gene in inducing multidrug resistance in human multidrug-resistant K562/A02 cell line, and search for a novel strategy for reversing multidrug resistance of leukemia cells.
METHODSThe expression levels of GCS and MDR1 mRNA in K562 and K562/A02 cells were assayed by RT-PCR. siRNAs targeting the GCS and MDR1 gene were transfected into K562/A02 cells with liposome, respectively. The differential expression of GCS and MDR1 mRNAs, as well as their correlation, were detected by RT-PCR and real time quantitative-PCR(QPCR).
RESULTSThe expression level of GCS and MDR1 mRNA was dramatically lower in drug-sensitive K562 cells compared with the K562/A02 cells. The GCS mRNA was inhibited by 73%(59%-82%) and MDR1 mRNA expression was down regulated by 67% (38%-82%) in K562/A02 cells after being transfected with GCS siRNA. The expression level of MDR1 mRNA was inhibited by 81%(63%-91%) and GCS mRNA expression had no apparent change in K562/A02 cells treated with MDR1 small interference RNA(siRNA).
CONCLUSIONPositive correlation was detected between the expression of GCS and MDR1 mRNA in K562/A02 cells and MDR1 mRNA expression was down regulated after silencing the GCS gene expression.
