Study of a novel compound 2460A with activities produced by fungus.
- Author:
Xiao-qiang QI
1
;
Feng-chang ZHU
;
Yang ZHANG
;
Lian-hong GUO
;
Rong JIANG
;
Qi-yang HE
;
Yuan LI
Author Information
1. Ministry of Health Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences & Peking Union Medical College, Bejing 100050, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
isolation & purification;
metabolism;
pharmacology;
Binding, Competitive;
Bone Marrow Neoplasms;
pathology;
Cell Cycle;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
HT29 Cells;
High-Throughput Screening Assays;
Humans;
Interleukin-6;
metabolism;
Ligands;
Receptors, Interleukin-6;
metabolism;
Trichoderma;
chemistry
- From:
Acta Pharmaceutica Sinica
2011;46(2):165-169
- CountryChina
- Language:Chinese
-
Abstract:
With IL-6R as target, a new compound 2460A was identified from fungus using HTS screening model. The taxonomics of the produced strain was confirmed to be Trichoderma hazianum rifai after sequencing analysis of rDNA-ITS (internal transcribed spacer). Results showed that this compound has a binding activity on IL-6R competed with IL-6, thus it is a new ligand of IL-6R originating from microbe. With MTT assay, the anti-tumor activities of 2460A were demonstrated on CM126 and HT-29 cell lines separately, the IC50 are 2.17 x 10(-5) mol x L(-1) and 1.8 x 10(-5) mol x L(-1) respectively. The compound affected lightly the HT-29 cell cycle at S phase. Studies for the anti-tumor activity of 2460A in vivo are in progress in our lab.
