Microcalorimetric study on the metabolism of Staphylococcus aureus affected by qingkailing injection.
- Author:
Cheng JIN
1
;
Yan-shu WU
;
Qian ZHANG
;
Xing-feng LI
;
Dan YAN
;
Du XIAO-XI
;
Xiao-he XIAO
Author Information
1. China Military Institute of Chinese Materia Medica, Military 302 Hospital, Beijing 100039, China.
- Publication Type:Journal Article
- MeSH:
Calorimetry;
methods;
Dose-Response Relationship, Drug;
Drug Combinations;
Drugs, Chinese Herbal;
administration & dosage;
isolation & purification;
pharmacology;
Plants, Medicinal;
chemistry;
Quality Control;
Staphylococcus aureus;
drug effects;
growth & development;
metabolism;
Thermodynamics
- From:
Acta Pharmaceutica Sinica
2011;46(2):193-197
- CountryChina
- Language:Chinese
-
Abstract:
This paper is to report the investigation on the metabolic behavior of Staphylococcus aureus (S. aureus) after given Qingkailing injection, and with the aim of seeking for a new quality control method based on biological assessment. The growth thermogenic curves of S. aureus were determined by microcalorimetry and analyzed by computer. The results showed that in the concentration range of (0-5.00%), the growth thermogenic curves of S. aureus were declined and removed back with increasing dosage of Qingkailing injection; the main parameters (T1, T2, k1, P1, P2 and I) and the dosage of Qingkailing injection have good correlation. The 50% inhibiting dosage is 3.26 %, and the optimal inhibiting dosage is 5.47%. Difference could be detected among the Qingkailing injection samples from different factories and different batches. It is proved that Qingkailing injection could inhibit the metabolic behavior of S. aureus, and microcalorimetry might be applied in the quality assessment of Qingkailing injection.
