The synergistic antitumor effects of berberine alpha-hydroxy-beta-decanoylethyl sulfonate with hydroxycamptothecine and its effect on topoisomerase.
- Author:
Jing LIN
1
;
Xi WANG
Author Information
1. Department of Pharmacology, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China. yq509@163.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
pharmacology;
Berberine;
analogs & derivatives;
pharmacology;
Camptothecin;
analogs & derivatives;
pharmacology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
DNA Topoisomerases, Type I;
metabolism;
DNA Topoisomerases, Type II;
metabolism;
Drug Synergism;
Humans;
Topoisomerase I Inhibitors;
pharmacology
- From:
Acta Pharmaceutica Sinica
2011;46(4):390-394
- CountryChina
- Language:Chinese
-
Abstract:
Synergistic antitumor effects of HB (berberine alpha-hydroxy-beta-decanoylethyl sulfonate, houttuyn berberine) with HCPT (hydroxycamptothecine), and its correlative mechanism were studied in vitro. MTT assay was employed to determine the cytotoxicity of HB combined with HCPT in tumor cells culture in vitro, IC50 and combination index (CI value) were used to evaluate the synergistic effects. The supercoiled DNA relaxation mediated by topoisomerase I & II was measured by agarose gel electrophoresis assay, and influence of HB was detected. The results showed that HB could inhibit the proliferation of tumor cells (SGC-7901, SW1116 and SW480) in vitro, and the inhibition ratio was increased, IC50 was reduced when combining with HCPT. CI value of the two drugs was less than 1 in HepG2, SW480, SGC-7901 and SW1116 cells. The lowest value was 0.447, 0.626, 0.161 and 0.178 in these tumor cells, respectively, further indicating HB has synergistic action with HCPT on suppressing tumor proliferation. The agarose gel electrophoresis assay showed HB can inhibit topoisomerase I & II activity of SW480 cells at the concentration of 2.0-8.0 mg x L(-1). HCPT is a typical inhibitor of topoisomerase I , the synergistic action between HCPT and HB on suppressing tumor proliferation is perhaps related to the congenerous inhibition of topoisomerase.
