The protective effects of SB203580 against mortality and radiation induced intestinal injury of mice.
- Author:
Jian-hui CHANG
1
;
Heng ZHANG
;
Fang-xia GUAN
;
Yue-ying WANG
;
De-guan LI
;
Hong-ying WU
;
Cheng-chun WANG
;
Chang-hui ZHOU
;
Zhi-bin ZHAI
;
Lu LU
;
Xiao-chun WANG
;
Qi HOU
;
Ai-min MENG
Author Information
1. Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical Collage, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
radiation effects;
Caspase 3;
metabolism;
Enzyme Inhibitors;
pharmacology;
Imidazoles;
pharmacology;
Intestines;
drug effects;
metabolism;
pathology;
Ki-67 Antigen;
metabolism;
Male;
Mice;
Mice, Inbred C57BL;
Pyridines;
pharmacology;
Radiation Injuries, Experimental;
metabolism;
mortality;
pathology;
Radiation-Protective Agents;
pharmacology;
Random Allocation;
Tumor Suppressor Protein p53;
metabolism;
Whole-Body Irradiation;
p38 Mitogen-Activated Protein Kinases;
metabolism
- From:
Acta Pharmaceutica Sinica
2011;46(4):395-399
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the protective effects of the SB203580 against radiation induced mortality and intestinal injury of mice. A total of 67 male C57BL/6 mice (20.0-22.0 g) were matched according to body weight and randomly assigned to one of three groups: control, total body irradiation exposure (IR, 7.2 Gy) only, and IR (7.2 Gy) + SB203580 (15 mg x kg(-1)). 30 days survival rate was observed in the experiment. In intestinal injury experiment, the expression levels of caspase-3, Ki67, p53 and p-p38 were assayed in the mice intestine crypts. The results showed that the 30 days survival rate was 100% (control), 0 (IR) and 40% (IR+ SB203580), separately. Compared to the IR groups, the positive cells of caspase-3, p53 and p-p38 in crypt cells decreased 33.00%, 21.78% and 34.63%, respectively. The rate of positive cells of Ki67 increased 37.96%. Significant difference was found between all of them (P < 0.01). SB203580 potently protected against radiation-induced lethal and intestinal injury in mice, and it may be a potential radio protector.
