Determination of diosgenin and ruscogenin in Radix Ophiopogonis by nonaqueous capillary electrophoresis.
- Author:
Bao-mei HUANG
1
;
Cheng-wei YAO
;
Qing-quan BIAN
;
Zhi-guo WANG
;
Jin-yuan MO
Author Information
1. Chemical Department of Mianyang Normal University, Mianyang 621000, China. hbm790117@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Diosgenin;
analysis;
Electrophoresis, Capillary;
methods;
Ophiopogon;
chemistry;
Plant Roots;
chemistry;
Plants, Medicinal;
chemistry;
Quality Control;
Spirostans;
analysis
- From:
Acta Pharmaceutica Sinica
2011;46(4):443-446
- CountryChina
- Language:Chinese
-
Abstract:
Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol x L(-1) Na2B4O7-HCl (pH 7.61) in 70% methanol. The applied voltage was 25 kV and detection potential was at +0.70 V. With these conditions, the components were successfully separated. The content of diosgenin in Radix Ophiopogonis was 0.018 mg x g(-1) and ruscogenin was 0.008 mg x g(-1). The average recoveries of diosgenin and ruscogenin were 102% and 99.2%, respectively. A new method of the quality control of diosgenin and ruscogenin in Radix Ophiopogonis is provided.
