Detoxication effect of water-soluble imprinted cross-linked chitosan on depleted uranium induced toxicity to renal cells.
- Author:
Xiao-fei ZHANG
1
;
Chao LI
;
Chang-qi ZHAO
;
Li-hong LIU
Author Information
1. College of Life Sciences, Beijing Normal University, Beijing 100875, China.
- Publication Type:Journal Article
- MeSH:
Antioxidants;
metabolism;
Apoptosis;
drug effects;
Cell Line;
Cell Survival;
drug effects;
Chelating Agents;
administration & dosage;
chemistry;
pharmacology;
Chitosan;
administration & dosage;
analogs & derivatives;
chemistry;
pharmacology;
Copper;
chemistry;
pharmacology;
Cross-Linking Reagents;
administration & dosage;
chemistry;
pharmacology;
DNA Damage;
drug effects;
Epithelial Cells;
cytology;
ultrastructure;
Humans;
Inactivation, Metabolic;
Kidney Tubules, Proximal;
cytology;
ultrastructure;
Microscopy, Electron, Transmission;
Uranium;
toxicity;
Water
- From:
Acta Pharmaceutica Sinica
2011;46(5):513-520
- CountryChina
- Language:Chinese
-
Abstract:
To investigate whether a series of water-soluble cross-linked chitosan derivates synthesized in the guide of imprinting technology could be used as a uranium chelating agent to protect cells exposed to depleted uranium (DU), the imprinted chitosan derivates with high UO2(2+) chelating ability were screened, and cell model of human renal proximal tubule epithelium cells (HK-2) exposed to DU (500 micromol.L-1) was built, chitosan derivates (400 mg.L-1 ) was added to test group and diethylenetriaminepentaacetic acid (DTPA, 50 mg.L-1) was added to positive control group. The results showed that three Cu2+ imprinted chitosan derivates had higher uranium chelating ability (>49 microg.mg-1) than chitosan and non-imprinted chitosan derivates. Compared to the cells exposed to DU only, survival of cells in group added chitosan derivates rose up significantly (increased from 57.3% to 88.7%, and DTPA to 72.6%), and DU intracellular accumulation decreased, membrane damage and DNA damage also eased. Among the imprinted chitosan derivates, Cu2+ imprinted penta dialdehyde cross-linked carboxymethyl chitosan (Cu-P-CMC) was the best, and better than DTPA. From ultrastructure observation, the DU precipitates of test group added Cu-P-CMC were most grouped in a big hairy clusters in a string together outside cells. It is possible that the DU-chitosan derivates precipitates are too big to enter into cells, and from this way, the DU uptake by cells decreased so as to detoxication.
