Simultaneous determination of 5 kinds of alkaloids in Kechuanning tablets by SPE-UPLC under different UV-vis wavelength.
- Author:
Yong-li LIU
1
;
Dong-mei LI
;
Li FENG
;
Hao YUAN
Author Information
1. Hebei Provincial Institute for Drug Control, Shijiazhuang 050011, China.
- Publication Type:Journal Article
- MeSH:
Alkaloids;
analysis;
Chromatography, High Pressure Liquid;
Codeine;
analysis;
Drugs, Chinese Herbal;
administration & dosage;
chemistry;
isolation & purification;
Ephedra;
chemistry;
Ephedrine;
analysis;
Morphine;
analysis;
Papaver;
chemistry;
Papaverine;
analysis;
Plants, Medicinal;
chemistry;
Pseudoephedrine;
analysis;
Quality Control;
Solid Phase Extraction;
Tablets
- From:
Acta Pharmaceutica Sinica
2011;46(5):581-585
- CountryChina
- Language:Chinese
-
Abstract:
The paper is to establish a method for simultaneous determination of 5 kinds of alkaloids in ephedra and poppy which are in Kechuanning tablets. Solid-phase extraction (SPE) was adopted in pretreatment, and a UPLC method with 2 different wavelengths had been developed: 210 nm for the detection of morphine, codeine phosphate, ephedrine hydrochloride and pseudoephedrine hydrochloride, and 251 nm for papaverine hydrochloride. The column used was Acquity UPLC BEH C18 (100 mm x 2.1 mm ID, 1.7 microm) with linear gradient elution using acetonitrile and 0.1% phosphoric acid. The flow rate was 0.4 mL.min-1, and the column temperature was 30 degrees C. The linear response range was 0.375 0 - 12.50 microg.mL-1 for morphine, 0.064 32 - 2.144 microg.mL-1 for codeine phosphate, 0.030 06 - 1.002 microg.mL-1 for papaverine hydrochloride, 1.126 - 37.52 microg.mL-1 for ephedrine hydrochloride, 0.287 8 - 9.592 microg.mL-1 for pseudoephedrine hydrochloride (r = 0.999 7). The average recoveries of these compounds were 99.26%, 100.6%, 95.29%, 100.1% and 97.48%, respectively. This is a more reasonable and credible method of quality control for Kechuanning tablets.
