Microbial glycosylation of cardamonin by Mucor spinosus.
- Author:
Jing XU
1
;
Li YANG
;
Shu-Juan ZHAO
;
Gui-Xin CHOU
;
Zheng-Tao WANG
Author Information
1. Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
- Publication Type:Journal Article
- MeSH:
Biotransformation;
Chalcones;
chemistry;
isolation & purification;
metabolism;
Glucosides;
chemistry;
isolation & purification;
Glycosylation;
Magnetic Resonance Spectroscopy;
Molecular Structure;
Mucor;
metabolism;
Spectrometry, Mass, Electrospray Ionization
- From:
Acta Pharmaceutica Sinica
2011;46(6):733-737
- CountryChina
- Language:English
-
Abstract:
Microbial transformation of cardamonin by Mucor spinosus (CGMCC 3.3450) in preparative scale resulted in the isolation of two new products. Their structures were elucidated unambiguously by ESI-MS, 1H NMR, 13C NMR and 2D NMR spectra analyses as 4-O-beta-D-glucopyranosyl-6-hydroxy-2-methoxychalcone (1, 4-GluC) and 6-O-beta-D-glucopyranosyl-4-hydroxy-2-methoxychalcone (2, 6-GluC), respectively. The time-course of biotransformation by M. spinosus showed that both 4-GluC and 6-GluC appeared on the 2nd day. The optimal biotransformation temperature was 28 degrees C, the optimal biotransformation time was 72 h and the optimal concentration for cardamonin was 40 mg x mL(-1). This is the first time for successful microbial glycosylation of cardamonin in present research.
