Advances in parallel artificial membrane permeability assay and its applications.
- Author:
Yi-Fan WU
1
;
Hui LIU
;
Jing-Man NI
Author Information
1. Institute of Pharmaceutics, School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
- Publication Type:Journal Article
- MeSH:
Absorption;
Animals;
Blood-Brain Barrier;
Cell Membrane Permeability;
Humans;
Intestinal Absorption;
Membranes, Artificial;
Permeability;
Pharmaceutical Preparations;
metabolism;
Skin Absorption;
Stomach;
metabolism
- From:
Acta Pharmaceutica Sinica
2011;46(8):890-895
- CountryChina
- Language:Chinese
-
Abstract:
With the development of drug discovery, more and more candidate compounds need to be studied. Methods that can screen compound rapidly received significant attention. Parallel artificial membrane permeability assay (PAMPA) as a powerful tool has been applied to drug studies. It uses an artificial lipid membrane to mimic the barrier for drug permeability studies. This article introduces the establishment and characteristics of PAMPA, as well as its applications in screening compounds. It can be used as models (e.g. predicting the ability of compound in gastro-intestinal absorption, blood-brain barrier transportation and skin penetration) by changing the component of artificial lipid membrane. PAMPA has advantages in high throughput selection of valuable compound with low cost, versatile, low dose, and good reproducibility.
