The 102 kb pigmentation (pgm) locus of Yersinia pestis isolated from Microtus brandti.
- Author:
Zhi-kai ZHANG
1
;
Dong-zheng YU
;
Jian-hua ZHANG
;
Rong HAI
;
Hong CAI
;
Jian-chun WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arvicolinae; microbiology; Bacterial Proteins; genetics; metabolism; China; epidemiology; Cloning, Molecular; DNA, Bacterial; Humans; Pigments, Biological; genetics; Plague; epidemiology; microbiology; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Sequence Analysis, DNA; Virulence; genetics; Yersinia pestis; genetics; pathogenicity
- From: Chinese Journal of Epidemiology 2003;24(4):291-295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo find out the differences between 102 kb pgm locus of Yersinia pestis isolated from Microtus brandti with of other types, and the characters of Yersinia pestis isolated from Microtus brandti caused by their makeup of the 102 kb pgm locus.
METHODS102 kb pgm locus of Yersinia pestis isolated from Microtus brandti and Yersinia pestis isolated from Marmota himalayana were amplified by polymerase chain reation (PCR) with 25 pair of nested primers. The PCR products of one pair of primer were obviously different, and then cloned and sequenced. Sequences were searched against current protein and nucleotide databases, using BLAST.
RESULTSThe 102 kb pgm locus of Yersinia pestis isolated from Microtus brandti was devoid one IS100. In addition, it had more copies than other types in the similar variable-number tandem repeat sequences.
CONCLUSIONThe 102 kb pgm locus of Yersinia pestis was different from that of other types. It had only one IS100 flanked it, which corresponded to the character that its pgm(+) phenotype was stable. Further study was needed to confirm the relationship between the diminution virulence of Yersinia pestis isolated from Microtus brandti and the loss of IS100 and other changes.
